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Testing methods

HPLC

Separating sample components for measurement. Reported purity depends on the method.

GlossaryUpdated 2026-09-24
01

Chromatographic separation

HPLC separates components for measurement with an appropriate detector.

02

Detector response

A trace shows signals over time. The method affects which components are detected.

03

Method-dependent purity

Peak-area purity is not automatically the target material's share of the vial's total mass.

The short answer

HPLC is high performance liquid chromatography. It separates sample components so they can be measured with an appropriate detector.

A reported chromatographic purity is method-dependent. It is not automatically the target material's percentage of the vial's total mass.

Reading the result

The chromatogram shows detector signals as peaks over time. A peak-area purity result compares the assigned target peak with other detected peaks under that method.

Keep the method in view

Detector response, separation and which components are detected affect the result. A purity percentage alone does not describe substances the method misses or establish measured milligram content.

What to ask for

Review the lot, method, result and supporting trace. Ask the laboratory to explain unclear peaks or calculations rather than judging a report from its headline number alone.

A useful next step

Read more than the headline percentage.

Review the lot, method, result and supporting chromatogram. Ask the laboratory to explain unclear peaks or calculations rather than inferring a result from appearance alone.

Compare purity and identity →
Continue reading

Read the COA guide

Continue with the supporting guidance. Research information does not establish legal clearance or clinical suitability.